Frequency of qnrA Gene among Fluoroquinolones Resistant E.coli Isolates from Urine of Imam Khomani hospital's patients

Back ground and This study was done to determine the recent resistance rate of E.coli strains isolated from urinary tract infections of p atients of Imam khomanin hospital to common antibiotics especially quinolons and screening the frequency of qnrA gene among them. During 6 months, 100 E.coli strains collected randomly from urine samples. All confirmed based on b acteriologic tests and their sensitivity to common antibiotics were determined based on CLSI 2011 protocol. Continuously, DNA extraction was done and frequency of qnrA gene was determined by PCR. Based on antibiogram, their resistant was 100% to amoxicillin and penicillin, 77% to amoxiclauve, 72% to ceftazidim, 69% to cephotaxim, 51% to ciprofloxacin, 47% to cefexim, 46% to ceftriaxone, 43% to cephalexin, 27% to axtreonam, 14% nalidixic acid and 2% to imipenem. Among 39.5% of E.coli isolated a 51 6bp band related to qnrA was detected by PCR. Doing antibiogram in all clinical laboratories as a simple and cheap test before any antibiotic prescription especially quinolons to prevent drug resistant is recommended.