Morphological and Genetic Characteristics of the Liver Hydatid Cyst of a Donkey with Iran Origin

No data is available on morphology and genetic characteristics of Echinococcus granulosus derived from donkeys of Iran, despite of its existence in don­keys. In the present study morphometric variations of the rostellar hooks of protoscole­ces and genotype characteristics of hydatid cyst of donkey from Iran were determined. Protoscoleces prepared from hydatid cyst of donkey of Iran were morpho­metric and genetic analyzed. The genetic analysis was done using Cox 1 gene by comparative sequence analysis. Our morphometric results showed that donkey of Iran shares 6 out of 7 determined parameters with donkeys of Jordan and 4 out of 7 with 4 available data with Switzerland donkeys. Morphological similarities and dissimilarities were ob­served with sheep-dog (G1) and camel-dog strains (G6) of Iran. The nucleotide sequence alignment showed that the partial sequence of Cox 1 from donkey had 91% homology with query coverage of 99% to the corresponding sequence of E. equinus, 90% homology to the E. felidis, 90% homology to E. ortleppi, 89% homol­ogy to the E. shiquinus, 89% homology to the E. vogeli, 89% homology to the E. oligarthrus, 88% homology to the E. canadensis and 83% homology to the Taenia so­lium. Additionally, the amino acid sequence of this gene has also some differences between this strain and all known strains of E. granulosus even with E. equinus (G4). Despite of common morphological characteristics of Iranian donkey hydatid cyst with those of donkeys of other parts of the world, genetically it has its own entity.