Increased trigonelline production by salicylic acid in Fenugreek (Trigonella foenum-graecum L.) cell culture

Manipulation of cell culture media by elicitors is one of the important strategies for inducing secondary metabolism production as valuable metabolites. Salicylic acid (SA) as a biotic elicitor is effective for increasing medicinal metabolites. The aim of this study was to evaluate the effectiveness of salicylic acid on the growth، some of physiological parameters and trigonelline production in fenugreek cell culture. The cell was obtained from hypocotyl explants of fenugreek on MS solid medium supplemented with naphthaleneacetic acid (0. 4 mg/L) and benzyladenine (0. 4 mg/L). Salicylic acid with concentrations of 12. 5 and 25 and 50 mg/L were used. After one week، some of physiological parameters and trigonelline production in fenugreek cell culture were investigated. Following treatments with salicylic acid، the results showed that the cell growth and viability of cells were decreased as compared to control. The treatments increased hydrogen peroxide content and lipid peroxidation rate in cells compared to control. In addition trigonelline and total phenol production were improved by all the treatments. Treatment of cells with 50 mg/L salicylic acid increased trigonellin 2- fold higher than control. Salicilyc acid can be used as an elicitor in fenugreek cell culture and induce more trigonelline production.