Diversity of culturable Moderate halophilic and Halotolerant Bacteria in Incheh Boroun hyper saline wetland in Iran

Incheh Borun hypersaline wetland is located near the border of Turkmenistan،in north of Iran. This wetland is remarkable because of salinity and variation in pH range. sampling was carried out from soil، water and salt in September 2010. 400 strains were purified and 55 strains were selected randomly for PCR. Genera: Bacillus (18%)، Marinobacter (16%)، Halomonas (16%)، Kocuria (9%)، Oceanobacillus (7%)، Dietzia (7%)، Virgibacillus (6%)، Chromohalobacter (5%)، Rhodococcus (2%)، Micrococcus (3%)، Paenibacillus (2%)، Halobacillus (3%)، Thalassobacillus (2%)، Arthrobacter (2%) and Desmospora (2%) were isolated. 13 strains showing 97- 98. 4 % similarity and 7 strains had less than 97% similarity in 16S rRNA sequencing which is showed significant differences in the level of species or even genus. Optimum growth for salt was evaluated and 22 strains were moderate halophile and 33 strains were halotolerant. Producing 4 hydrolytic enzymes was evaluated which the main producers of hydrolytic enzymes were Gram-positive bacilli and the most frequent enzyme was Gelatinase and Protease.