Study of polymorphism in Mazandaran and Esfahan native chicken population using microsatellite markers

In order to studying of genetic variation in Mazandaran and Esfahan native chickens، twenty microsatellite markers were evaluated. These microsatellite markers were MCW0014، MCW0081، MCW0183، MCW0067، MCW0104، MCW0123، MCW0330، MCW0165، MCW0069، MCW0020، MCW0222، LEI0094، MCW0295، MCW0034، MCW0216، ADL0268، ADL0112، ADL0278 and lEI0166. Blood samples of 90 and 150 native chickens of Mazandaran and Esfahan were randomly taken respectively. Genomic DNAs were isolated through optimized and modified salting-out procedure. The number of alleles varied from 1 to 6. In Mazandaran population one locus (MCW0216) and in Esfahan population three loci (MCW0216، MCW67 and MCW222) were monomorphic. The other loci were showed appropriate polymorphism. All the loci except MCW222 and MCW165 in Mazandaran population showed deviations from Hardy-Weinberg equilibrium (p)