The Effect of Apium graveolens hydroalcoholic Seed Extract on Sperm Parameters and Serum Testosterone Concentration in Mice

Backgrounds & apium graveolens contains antioxidant activity and high level of polyphenolics. The purpose of this study was to determaine the effect of Apium graveolens seeds extract on semen parameters and serum testosterone level in mice. In the present experimental study, sixty male mice were divided into three experimental groups and a control group. The hydroalcoholic seed extract of Apium graveolenas L. was administered intraperitoneally at the doses of 200, 400 and 800 mg/kg for 14 days. A week after the final injection, blood samples were collected for hormonal assay. Then, the testes weight, sperm count and cauda epididymal sperm motility was assessed. Data were analyzed by ANOVA and Tukey's test. The results were compared with the control group indicating a significant increase in the total number of sperm at dose 400 mg.kg and increase sperm motility was seen in groups receiving 200 and 400 mg.kg respectively (P<0.001). Increased testosterone levels in the group receiving 400 mg.kg compared with the control group was observed (P<0.01). A significant increase was seen in testes weight compared with the control group (P<0.05). Apium graveolens seed extract appeared to be effective in improving semen parameters and serum total testosterones were dose dependent.