Genetic structure of spirlin (Alburnoides echiwaldii) in Karganroud and Chalous rivers

Genetic structure of two populations of Spirlin، Alburnoides eichwaldii from Karganroud and Chalous Rivers was investigated using six microsatellite molecular markers (CtoF-172، BL1-2b، CnaB-030، LleA-071، Ca3 and Z21908) on 30 fishes from each river. The mean of observed alleles at each locus was 6. 5. Allele sizes at CtoF-172، BL1-2b، CnaB-030، LleA-071، Ca3 and Z21908 loci were ranged from 107-147، 147-184، 124-157، 332-387 300-347 and 147-183 bps respectively. Z21908 and Ca3 showed the lowest and the highest polymorphism respectively. All loci in both rivers showed deviations from Hardy-Weinberg equilibrium. The number of effective alleles، expected heterozygosity، observed heterozygosity and polymorphism information content (PIC) were 4. 86، 0. 81، 0. 96 and 0. 94 respectively cindicative of high level of genetic diversity in both populations. The mean FIS value for all loci in both station ranged from -0. 23 to -0. 30 indicating low possibility for inbreeding occurrence. The analysis of molecular variance (AMOVA) indicated that the percent of variance among and within populations were 6. 31 and 93. 69 %، respectively. The genetic distance was 0. 363. Significant FST value (0. 063) was observed between the two populations. The high level of genetic differentiation may reflect local segregation of two populations because of differences in ecological conditions of two rivers.