Isolation and purification of spermathogonial stem cells from adult goat testis

The Spermatogonial Stem Cells (SSCs) are founder of spermatogenesis and they are the only stem cells that can transmit genetic materials to offspring’s. This research was conducted to achieving a reliable origin of SSCs from the goat testicular tissues according to a four steps procedure; a) Collection and preparing testicular tissues، b) Two enzymatic digestions، c) Using mesh and cell suspension and d) Culture the isolated SSCs on goat embryonic fibroblasts. ِDistinct markers of pluripotency such as NANOG، SOX2and OCT4 were expressed in the isolated SSCs and also in these cells، nonspecific alkaline phosphatase test was positive. These cells used for co-culturing with GEF for several passages without differentiation or changing the shape. It was also found out that by using DMSO، these cells can be held in -70°C for one month. The result showed that the used procedure was efficient and the cells not only can express distinct markers of pluripotency but also can be culture and proliferate for long period of time without differentiations.