Identification and determination antimicrobial resistance pattern of Haemophilus influenza isolated from patients with otitis media

Otitis media is one of the most common infectious diseases in children. Approximately 85% of children will have otitis media at least once by the age of 3 months, and nearly 50% of children would have more than three times. The objective of the this research was to detect Haemophilus influenza, the most common cause of otitis media in children, and to determine the antibiotic susceptibility pattern of this organism. In this cross-sectional study,a total of 102 specimens were collected during 6 months period from patients with otitis media referred to Amir Alam Hospital in Tehran.Specimens were evaluated for Haemophilus influenzae by microscopic examination and culture. The antibiotic susceptibility test was performed by disk diffusion method of Kirby-Bauer and sensitivity rate of tested antibiotics were assessed by evaluation diameter of the growth inhibitory zone. Out of 102 tested specimens, 7 (6.9%) were identified as Haemophilus influenzae. The rate of sensitivity of isolated Haemophilus influenzae to different antibiotics was trimethoprim (100%), vancomycin (85%), streptomycin (57%), tetracycline (57%), and doxycycline (56%),and the rate of resistance to gentamycin (85%), carbenicillin (70%) and cefixime (70%), clindamycin (70%) amikacin (57%) amoxicillin (57%). With respect to appearance of new resistance species especially to clinidomycin, amikiacin and amoxicilin it is recommended to perform antibiotic susceptibility tests before the initiation of treatment.