Evaluation of natural autoantibody against red blood cells in healthy individuals

Natural autoantibodies (N-Auto-Abs) are a class of antibodies that are formed without any previous provocation against their own. Currently an important role has been proposed for these antibodies in immune homeostasis; as we can study them as biological markers to predict the onset of the disease. For the first time, we studied the prevalence of N-Auto-Abs against RBC antigens as well as the prevalence of cold antibodies in blood donors. hree hundred healthy individuals who donated at Tehran Blood Transfusion Center were selected randomly; their blood samples were collected including 289 (96.3%) males and 11 (3.7%) female. Their age range is within 17-64 years. EDTA plasma was evaluated for the study of N-Auto-Abs that reacted at 4C. 28 individuals (9.34%) showed positive reaction with their own cell suspension. In addition, 55 individuals (18.34%) were positive for cold antibodies. Twenty eight individuals (9.34%) showed positive reaction with their own cell suspension. In addition, 55 individuals (18.34%) were positive for cold antibodies.