Effect of Intensive Resistance Exercise Training on Protein Expression of IL-6, IL-1β and TNF-α myokines in fast twitch skeletal muscle of diabetic rats

It suggested that cytokines or other peptides that are produced, expressed and released by muscle fibres (usually during contraction) and exert endocrine effects should be classified as myokines. Many proteins produced by skeletal muscle are dependent upon contraction. Therefore, it is likely that myokines may contribute in the mediation of the health benefits of exercise. Our aim was to investigate effect of intensive resistance exercise training on protein expression of IL-6, IL-1β and TNF-α myokines in skeletal muscle in rats with diabetes. Rats were divided into control, training, streptozotocin induced diabetic and diabetic training groups. Training groups performed for Resistance training consisted of climbing a 1 m ladder with increasing weight added to the tail. Protein expression of myokines in FHL skeletal muscle measured by ELIZA method. The results of this study indicate that training and diabetes interaction was significant for IL-6 in FHL muscle (P<0.05). Also, tumor necrosis factor-alpha (TNF-α) and IL-1β levels were increased by diabetes, but not changed by training. Discussion and In conclusion, we found that in diabetic rats, resistance training decreased muscle IL-6 levels, and preserved FHL muscle mass. It is probably increased inflammatory cytokines in skeletal muscle with resistance training is a mechanism for skeletal muscle adaptation.