Purification of lipase from the foregut of Rainbow trout (Oncorhynchus mykiss)

Aquatics have been considered as resource for extract of enzymes such as lipase in past years. Viscera of rainbow trout are a very good resource for the isolation of enzyme. In this study، lipase enzyme was purified from the foregut of rainbow trout. For purification، was used the acetone to defat، ammonium sulphate for precipitation، ultrafiltration to concentration and chromatography of gel filtration in order to isolation. Enzyme activity with Nitrophenyl palmitate as substrate and protein of this enzyme by Lowry method were assessed in the different steps of purification. In this study، parameters such as purification efficiency and the purified was examined. The results showed that in the last step of purification، enzyme activity and enzyme protein were 6. 171 U/ml and 0. 53 mg/ml، respectively، purification efficiency was 7. 53% and the purified was 5. 49. The molecular weight of lipase was determined by SDS-PAGE 32 KDa. Based on this research it can be concluded that the lipase in the intestines of rainbow trout has been useful as an additive in the future can be considered related industries and in Biotechnology Research.