Synthesis and Optimization of Human Serum Albumin Nanoparticles for Drug Delivery Application

In recent years, the application of protein nanoparticles has become of more and more interest to the pharmaceutical industry. In this study,Human Serum Albumin (HSA) was used for the synthesis of nanoparticles by desolvation method for application in drug delivery system because of biodegradablity, owing to the high capacity of drug loading and nontoxiticty. Moreover, the effects of different parameters i.e. pH, HSA concentration, agitation speed, glutaraldehyde concentration, organic solvent adding rate, the ratio of organic solvent/HSA solution were examined in this research and Taguchi method with L16 orthogonal array was implemented to optimize experimental conditions. The best parameters for nanobioparticles production were obtained at pH=9, HSA concentration: 75 mg.ml-1, ethanol adding rate: 1.5 ml.min-1 and the ratio of organic solvent/HSA solution: 4. Under these conditions, the software predicted the 46.625 nm for HSA nanoparticle size, which, in experiment 53 nm, was achieved for the nanoparticles. With respect to our study, the synthesis of human serum albumin nanoparticles was carried out for the first time and to the best of our knowledge, no reference has been found in the literature about using Taguchi method for optimization of HSA nanoparticles up to now.