Therapeutic Effect of Allium cepa, Oliveria decumbens Vent and Muscari neglectum against Trichomonas vaginalis, in Vitro

Trichomonas vaginalis is a flagellate pathogenic protozoa of human urogenital tract, that infect 170 million new cases through the word annually. Considering to the side effects of metronidazole as common drug to treatment of this disease, this study was conducted to evaluate the in vitro activity of extract of Allium cepa, Oliveria decumbens Vent and Muscari neglectum against Trichomonas vaginalis. This experimental study was carried out on Trichomonas vaginalis isolated from patient referred to five Kashan, Iran health centers in 2013. The effect of alcoholic extract of Allium cepa, Oliveria decumbens Vent and Muscari neglectum in 12.5, 25, 50, 100, 200, 400, 800and 1000 μg/ml concentration was evaluated on growth of Trichomonas vaginalis in TYI-s-33 medium after 12,24and 48 hours. The results was compared with metronidazole effect in 0.025, 0.05, 0.1, 0.2, 0.4 μg/ml concentration as positive control. The number of alive and dead throphozoite measured and compared by trypan blue staininig. IC50 was determined using GraphPad prism5. This study showed that the alcoholic extract of Allium cepa, Oliveria decumbens Vent and Muscari neglectum has inhibitory effect on invitro growth of Trichomonas vaginalis. Effect on growth of Trichomonas vaginalis in vitro condition. The IC50 rate was calculated 101.8 for Olivera dacumbens vent, 572.3 for Allium cepa and 329.4 for Muscari neglectum after 24 h. The IC50 rate of mrtonidazole was calculated 0.0326 as the same time. Alcoholic extract of Allium cepa, Olivera dacumbens vent and Muscari neglectum has antitrichomonal effects. The effect of Oliveria decumbens Vent was higher than others, we recommend further studies using major components of these plants especially Olivera dacumbens, to investigate the antitrichomoniasis effect of it.