Effects ofMelissa officinalisExtractonLiver Enzymes and Histologyin Mice

Balm (Melissa officinalis) has many medical properties including relaxing، anti oxidant، and anti bacterial. This study was conducted to investigate the effect of hydro alcoholic extract of balm on activity of liver enzymes including AST، ALT، and ALP and also liver tissues variation in Balb/C mice. In this experimental study، samples were randomly divided into fivegroups (three treatment groups، placebo and control group) with eight members in each group. Groups were kept under similar conditions. Hydro alcholic extract was prepared in three doses (50،100، and 200 mg/kg) and were IP for twenty days. Normal saline was used for placebo group. After the last injection blood samples were taken and liver tissues were separated. Changes of AST، ALT، and ALP enzymes was evaluated using one way variance analysis and SPSS program (P<0/05). Tissue changes of liver were studied using coloring with H&E technique. According to results، ALT، and ALP enzyme was increased (P<0. 05) in 200 mg/kg dose. Hepatocytes number and histology of liver didn’t show significant variations. On the whole، hydro alcoholic extract of balm could have conservative effects via reducing ALT and ALP enzymes and no harmful effects on liver tissue. So، it can play an important role in preventing and curing diseases in traditional and also current medicine.