Correlation between the expression of OprD gene and sensitivity to carbapenems of Pseudomonas aeruginosa strains isolated from clinical samples using qRT-PCR

Pseudomonas aeruginosa is one of the most common nosocomial pathogens with high mortality rates. OprD is the major resistance mechanism to carbapeneme antibiotics. The aim of this study was to determine the expression of the genes encoding these efflux pumps using qRT-PCR. This study examined 100 strains of Pseudomonas aeruginosa isolated from patients admitted to various hospitals in the Hamedan. Conventional phenotypic tests were used for identifying the 100 collected samples, then 31 samples were selected based on type of collected specimen and antibiotic susceptibility test i.e. antibiotic disk diffusion method performed for aminoglycoside, quinolone and carbapenem antibiotics. Furthermore, MIC method was performed for imipenem. Finally, RNA was extracted and converted to cDNA for determining the efflux pump genes expression using qRT-PCR. Among 8 selected antibiotics, the greatest resistance was for levofloxacin (61.2%, n=19) and the lowest one for imipenem (9.6%, n=3). The results of MIC were to imipenem 12 samples (38.7%) resistant, 13 samples (41.93%) intermediate, and 6 samples (19.35%) sensitive. The OprD gene was present in all strains but different expression has been observed. The strains with over expression of OprD gene showed high sensitivity towards carbapenems family antibiotics especially imipenem. Identifying of bacterial resistance mechanisms is very complicated and extensive due to different mechanisms involved for similar antibiotics. OprD is main cause of attachment to the carbapenems family antibiotics. The more expression of OprD shows the more antibiotic sensitivity.