Effect of of α-linolenic acid in maturation medium on developmental competence and quality of blastocysts derived from parthenogenetic activation/fertilization in vitro
Author(s):
Abstract:
In vitro maturation (IVM) was carried out in the presence of different concentrations (10، 50، 100 or 200 µM) of α-linolenic acid (ALA). Embryonic cleavage، blastocyst formation following parthenogenetic activation (PA) and in vitro fertilization (IVF)، and numbers of total and apoptotic cells in blastocyst were then determine for the 50 μM concentration and compared with the control group. Out data revealed that ALA increased maturation (MII) rate as compared with control group (P<0. 05) and oocytes in 200 μM ALA group showed a lower MII rate as compared with the control group. When oocytes treated with 50 μM ALA were subsequently used for PA or IVF، a higher (P<0. 05) rate of blastocyst formation was observed and these embryos had a higher total cell number and a lower apoptotic cell number (P<0. 05) as compared with the control group. In conclusion، our results show that supplementation of maturation medium with 50 μM ALA had a positive effect on meiotic maturation by increasing the MII rate and this in turn، stimulated blastocyst formation and also improved quality of the yielded blastocysts.
Keywords:
Apoptosis , blastocyst , goat oocyte , α , linolenic acid
Language:
Persian
Published:
Journal of Animal Productions, Volume:16 Issue: 2, 2015
Pages:
75 to 83
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