Diversity in Helicobacter pylori by Multi Locus Sequence Typing

Helicobacter pylori as an etiological agent of active chronic gastritis and peptic ulcer disease، is now considered to be an important pathogen for gastroduodenal diseases. Resistance to antimicrobial drugs is a major cause of treatment failure and decline in eradication rates in most of countries. The aim of this study was molecular typing of Helicobacter pylori isolates was done throughout MLST (multi locus sequence typing)، the most reliable typing method، which is based on DNA sequencing. H. pylori isolates of gastric biopsies from patient''s ≥ 18 years old were used in this study. Genomic DNA was extracted and used for PCR of glmM، atpA، mutY، efp، trpC، yphC، ppa and ureI. PCR product of 7 latter genes sequenced and analyzed with proper bioinformatics soft wares and compared with other H. pylori isolates from other countries. According to MLST results the isolates were so divers and different from other countries. According to different STs resulted from MLST typing، it can be considered that H. pylori isolates in Iran are very different. It may due to many DNA exchange mechanisms which H. pylori have.