Evaluation of toxicogenic potential of Aspergillus tubingensis isolates from grape and raisin in southern regions of East Azarbaijan province

Toxicogenic potential of Aspergillus tubingensis isolates occurring on grape and raisin in southern regions of East Azerbaijan province were evaluated using of ammonia vapor, UV photography and high performance liquid chromatography (HPLC) methods. For this purpose, fungal isolates were grown on Capek Yeast Autolysate Agar (CYA) and Coconut Cream Agar (CCA) media and Ochratoxin A (OTA) production was evaluated. The results of this study showed that toxin production was detectable on 69%, 41% and 65% of the isolates using ammonia vapor, UV photography and HPLC methods, respectively. The compatibility of ammonia vapor vs. HPLC, HPLC vs. UV, ammonia vapor vs. UV and three mentioned methods was 50%, 69%, 62% and 42%, respectively. According to the results of this study, UV photography method appeared to be more efficient for detection of toxigenic isolates of A. tubingensis in comparison to ammonia vapor. The amount of OTA produced by isolates was determined 29-1089 μg/kg using HPLC.