The effect of NAA and different explants on Secondary somatic embryogenesis of strawberry (Fragaria ananassa Duch.)

The present investigation was conducted to study the effects of different concentrations of NAA on Somatic embryogenesis induction, development and maturation of three strawberry (kurdistan, parose and camarosa) cultivars. For this purpose, leaf blade, nodal, petiole, stamen and flower bud calli were cultured on Murashige and Skoog (MS) medium supplemented picloram at 0.25, 0.5, 1 and 2 mg/L. The concentration of growth regulator, cultivar and explant type were found critical to Somatic embryogenesis induction, development and maturation. Results obtained from the studies revealed that all explants exception of petiole and stamen incubated on medium formed embryonic calli. MS medium supplemented 1mg/L NAA yielded the highest percentage of embryonic calli and number of embryos globular stage and 0.5 mg/L NAA yielded the highest Number of embryos cotyledonary stage in all types of explants. The leaf explant calli and paros cultivar were the most responsive to produce to Somatic embryogenesis induction, development and maturation.