Preparing Microsom and Measuring it's Protein in human liver samples

Background and In human cytochrome P450 (CYP), enzymes are responsible for the oxidative metabolism of many medicines. The greatest metabolism proportions are carried out by enzymes that are found in the liver. CYPs are located in the microsomes. For these reasons preparation of microsomes and measurement of their protein are very important to determine the potency of liver for metabolic elimination of drugs in humans. The aim of this project was to extract microsmes and determine levels of microsomal protein in human tissue. Eighteen biopsies of human liver were obtained with their consents from patients undergoing surgery. Liver samples were finely chopped and homogenized in the buffer. Liver homogenate was centrifuged in different steps to yield a microsomal pellet. Then content of total protein was determined according to Lowry method. Data were analyzed according to descriptive statistics method. The value of MPPGL (microsomal protein per gram of liver) ranged from14.1 to 27.2 mg per gram of liver (mean 20.24±3.77). The values of MPPGL in this project were reported for the first time in Iran. The mean value of MPPGL in this project is lower than others studies. These results in this area, will aid the physicians to improve the level of safety of prescription of the medicines. Many doctors are unaware of the possible risks for patients whom have exposed by being treated with drugs.