Ovarian histological and sexual maturation study in bream Abramis brama orientalis (Berg, 1905) through induce LHRHa2 and dopamine antagonists

In the present study histological studies was conducted after the female Abramis brama orientalis was exposed to eight treatment phases in which LHRHa2 (3 μg/kg)، Metoclopramide (5 mg/kg)، and Chlorpromazine (10 mg/kg) were inoculated. The treatments were characterized by combined and solitary injections، control group (o injection)، and experimental group (saline). The results showed that all three investigated compositions enjoy arousal effects which bring about sexual maturity. In both solitary and combined treatments LHRHa2 entailed oocyte maturity and qualitative developments among the oocytes occurred during the spawning period and fat vacuole as a macro vacuole and resolution of the vitelline took place due to improvements in the proteolitic activity during the final maturity phase. Metoclopramide fostered sexual maturity among the oocytes which have been ready to enter the last and final sexual maturity stage; however، Chlorpromazine did not have any significant impacts and thereby they are not identified as a powerful and special dopamine types. Oocyte diameter in treatments Metoclopramide: 810μm، LHRHa2:773μm،Chlorpromazine: 680μm،Metoclpramide+LHRHa2:545μm،Chlorpromazine+LHRHa2:515μm،Metoclopramide+Chlorpromazine+LHRHa2:383 μm and Control: 379.