A radiographic survey of mandibular bone defect healing by combination of platelet-rich-plasma (PRP) and nanohydroxyapatite in dog

Nanohydroxyapatite is an osteoconductive agent which has recently been used in some research as a bone graft. It has been showed that these particles increase the rate of bone repair due to advantages such as high purity، having more connections with surrounding cells، faster absorption and greater number of molecules per unit area than conventional hydroxyapatite. On the other hand، platelet-rich plasma is used as a source of osteoinductive factors for bone repair. Thus، the present study was performed to evaluate the efficacy of nanohydroxyapatite and platelet-rich gel combination on healing bone defects. For this study، 15 male dogs were considered. Six circular defects of 5 mm in diameter was created on both sides of mandible and then each defect was filled with one of the following material: conventional hydroxyapatite، nanohydroxyapatite، platelet-rich gel، hydroxyapatite and platelet-rich gel combination، nanohydroxyapatite and platelet-rich gel combination and finally a defect was excluded as the control group. Evaluation of defect healing was performed with radiographs on days 0، 7، 14، 21، 28، 35، 42، 49 and 56. The results showed that nanohydroxyapatite and platelet-rich gel combination can heal faster than nanohydroxyapatite and especially platelet-rich gel alone، but the differences were not significant. Probably، this combination can be considered as an alternative to fill the bone defect. Further studies are needed to substantiate this claim.