The study isoforms and activities of antioxidant enzymes in immature flora segments of saffron (Crocus sativus L.) Iran

Saffron (Crocus sativus l.), member of Iridaceae which was used as spice and also in medicine. An investigation was set up in order to select suitable samples to improve saffron production. The activities and isoforms of antioxidant enzymes which were affected on H2O2/O2 ratio, that represented the potential of organogenesis was carried out by using electrophoresis and five different segments of saffron immature flora. The number of peroxidase isoforms which were specific-tissue and developmental stages that consumer of H2O2 and Superoxide dismutase which produce H2O2 from superoxide showed high ratio in ovary and low in perianth segments. Catalase was repressed by higher but responses by lower level of H2O2, acts versus peroxidase. The other Three enzymes, Polyphenoloxidase is specific-growth and represent defense system, Malatedehydrogenase, showed relationship between organs and their growth and also produced H2O2 and Estrase was specific- organization of tissue all gave us useful information to select right segments for our propose.