Investigation of Genetic Diversity in Chickpea (Cicer arietinum L.) Lines Using ISSR Markers

In this research، ISSR technique was applied to assess genetic diversity among 56 chickpea lines. Out of 34 primers، nine primers produced appropriate banding patterns and produced 35 polymorphic bands in chickpea lines. Mean percentage of polymorphism of the primers was 66. 01 with range of 25 to 100 percent. Mean of Nei''s genetic diversity and Shannon information index over all primers were 0. 325 and 0. 492، respectively. Based on Jaccard''s similarity coefficient، lines of FLIP01-40C and FLIP02-84 had the lowest similarity (0. 166) and lines of FLIP03-6C and FLIP03-8C had the highest similarity coefficient Cluster analysis using UPGMA method and Jaccard''s similarity matrix was done. The dendrogram was cut at 0. 51 scaled interval and categorized the lines in six distinct groups. Distinct grouping and large genetic distances among the chickpea lines stated a good molecular variation. Showing the lines by a tri-plot based on the three first coordinates from PCoA، verified the clustering and efficiently separated them.