Identification of Quantitative Genes of Protein Content and Gelatinization Temperature in Recombinant Inbreed Lines of Cross of Anbarbu×Sepidroud

Identification of controlling genomic loci of complex traits with providing of useful genetic information، as introduction of breeding programs of marker assisted selection are a practical approach in breeding of complex traits. In the present study، 96 recombinant inbred lines were used as mapping population to identify controlling QTLs of two important traits related to grain quality including of gelatinization temperature and protein content. Linkage map was constructed with 438 markers including 314 AFLP and 124 SSR. The results of composite interval mapping totally five QTLs detected for two traits. Between two identified QTLs for gelatinization temperature، qGT-6 was detected in vicinity of wx gene on the chromosome 6. Considering of Anbarbu allele’s role in both of QTLs in enhancing of gelatinization temperature، these QTLs can be very efficient in improving this trait. As well as for protein grain three QTLs was identified that considering of positive additive effect of qPR-1b and explain of more than 13 percent of phenotype variation it can be considered in marker assisted selection.