Morphological and Molecular Identification of Peyronellaea glomerata as Causal Agent of Leaf Spot of Field Bindweed in Kurdistan Province

During the autumn of 1392 strawberry farms were inspected in the province of Kurdistan. In most fields، leaf spot symptoms of field bindweed were observed. Infected tissues surface sterilized with 5. 0 percent sodium hypochlorite and were cultured on the medium PDA (potato، dextrose، agar). After seven days of incubation at 25-23°C، purifiction was done from edge of colonies in the maner of hyphal tip on water-agar medium. A total of 21 isolates posses pycnidium obtained that were morphologically similar. one of the isolates as representative was choosen and its ITS1، ITS2 and 5. 8s gene was sequenced. Finally، based on the results of morphological and molecular studies the causal agent of leaf spot of field bindweed was diagnosed as Peyronellaea glomerata. This is the first report of P. glomerata leaf spot on field bindweed. This fungus can be used as biocontrol agents in the integrated control of field bindweed if does not able to disease other crop in Kurdistan area.