Investigate the possibility of Sex determination of rainbow trout (Oncorhynchus mykiss) using molecular markers

The study investigates the possibility of sex determination of rainbow trout (Oncorhynchus mykiss) using molecular screen for products monosex of populations. 35 caudal fin tissue samples were obtained from Karaj Rainbow trout mature population and then genomic DNA was extracted and marker loci amplified. Polymerase chain reaction (PCR) was setup based on two omyFA and OmyFATU markers for amplification of sex linked markers in rainbow trout. Finally، the population was screened based on presence of a specific band for both markers. Result of this expriments showed that presence of omyFA and OmyFATU markers in males were %100 and %55 respectively. Acording to the result omyFATU is proper marker for screen of karaj trout population.