Effect of combined biologic and priming on growth characteristics and phosphorus and potassium concentrations in Nigella sativa L.

In order to study of the effect of combined biologic and priming on growth characteristics and phosphorus (P) and potassium (K) concentrations in Nigella sativa L.، an experiment was carried out in the greenhouse of Agriculture Faculty، University of Birjand with factorial CRD statistical design in 2011. Factors were 1- Bacteria treatments a (Pseudomonas sp 168)، b (Pseudomonas sp 187)، c (compose of Pseudomonas sp 168 and Pseudomonas sp 187) and d (without Bacteria treatment)، 2- priming with KH2PO4 (50 mM concentration) and without priming. Soil sample was taken from Bidokht village and sieved through a 4-mm and transported to pots of 3 kg (pH=7. 8 and ECe= 1 dS/m). Pots were irrigated based on field capacity moisture. Results showed that biologic treatments caused، significant increase in shoot dry weight and both P and K concentrations of shoot. Priming had excessive effect of biologic fertilizer on those of two elements concentrations in shoot of Nigella sativa L. in sixth week and shoot dry weight as well. Highest shoot concentration of K and P was observed in (Pseudomonas sp 168+priming) and (Pseudomonas sp 168+187+priming) respectively. Therefore، combine application of priming and biologic seed treatments can be useful method for increasing of P and K concentration in medicinal plants.