Study of Hepatoprotective Effect of Malva neglecta L. Hydroethanolic Leaf Extract in Male Rat Induced with Carbon Tetrachloride

In this study the hepatoprotectivity effect of Malva neglecta hydroethanolic extract (MHE) were investigated in male rats. 35 male rats with 230-250 gr weight were divided in 5 groups: control (taking normal saline,0.5ml/day, IP), sham(taking olive oil, 0.5ml/day,IP),carbon tetrachloride 1:1 with olive oil,0.5ml single dose, IP), treated 1&2 (carbon tetrachloride 1:1 with olive oil,0.5ml single dose and 300mg/Kg and 600mg/Kg MHE /day for 4 days, IP). After the end of examination the blood samples were collected from heart directly and AST, ALT and AP enzymes were analyzed. The microscopic studies of hepatic tissue were done and histological changes observed by (H&E) staining. All data analyzed with ANOVA test. Statistical significance was accepted at P<0.05. The serum liver enzymes were increased in rats induced by ccl4 compare to the control group (P<0.001) and decreased significantly in rats by treated MHE (P<0.01). Histological finding revealed the necrosis in hepatic tissue. In animals treated with MHE the results showed that the reduction in necrotic cells and increase in hepatocytes regeneration. Our results showed that the ccl4 has hepatotoxicity effect and it can improve inflammation and necrosis in liver tissue. The MHE have antioxidant and flavonoid that can decrease toxic effect of carbon tetrachloride in liver tissue.