Effects of medium, genotype and plant growth regulators on micro propagation of Jatropha curcas

Jatropha curcas is a tropical fast growing tree from euphorbiaceae family. The valuable species has medicinal، industrial and ornamental applications. Regeneration of the species was performed by seeds and cuttings. Regarding Jatropha''s seed nature (oily seed)، for which low germination is resulted، regeneration of the species was studied by stem cuttings. For micro propagation، apical buds of 2 and 4 years old genotypes̉̉̉ (Gen 1 and Gen 2) from different seasons were applied. The best treatment of sterilization was found to be HgCl2 0. 1% solution for 10 minutes on fall samples for genotype 2. The best medium for shoot proliferation was MS medium with BA (3 mg/l) and IBA (0. 1 mg/l). Effect of genotype on shooting was nonsense but genotype 2 had higher effect on growth factors. The most rooting of shoots was observed in modified MS medium supplemented with 1 mg/l of IBA. Plantlets were acclimated in greenhouse after transferring to soil.