Effects of culture medium and growth regulators on micropropagation of Eucalyptus occidentalis

Eucalyptus occidentalis is cultivated for its nectar and pollen in honey production، also due to its essential oils for medicinal aspects، tanin in leather industries and wood fibers in cosmetics and paper industries. Micropropagation of the species was evaluated by auxiliary bud culture in MS and GD culture medium using a factorial experimental model based on a completely randomized design. Seeds were immersed in water for 2 hours and 1% sodium hypoclorite solution for 18 minutes as the best treatment for surface sterilization. A modified MS medium containing cytokinines BAP، GA3 and IBA in 0. 5، 0. 15، 0. 01 mgl-1، respectively and 200 mgl-1 PVP was used. The best rooting treatment was a modified MS medium (1/2N) containing auxine، IBA in 2mgl-1. Plantlets were transferred to soil and kept in greenhouse conditions. The study showed that using an appropriate treatment in tissue culturing method، the valuable species could be propagated in a short time.