Karyotyping studies and Chromosome morphology in Mazani and Azeri buffaloes

In the present study karyotype of Mazani buffalo was studied in comparison with those of Azeri buffalo populations from Iran. Karyotyping analysisis a powerful instrument to determine the karyotype of farm animal and to turn up more about fundamental basis for chromosomal abnormalities.Blood samples were taken from ten (5 males and 5 females) Mazani buffaloes and thirty (15 males and 15 females) Azeri buffaloes. The Mazani buffaloes belong to state of Mazendaran and Azeri buffaloes belong to states of west and east Azerbaijan and Ardebil. Blood lymphocytes cultured at 37ºC for 72 hours in the presence of phytohemagglutinin and the metaphase spreads were performed on microscopic slide. Giemsa was used to stain chromosomes. The Mazani and Azeri Buffalo exhibited the same karyotype with diploid number of 2n = 50. The fundamental numbers (NF) were 60 in male and female. Stebbins classifications show both Mazani and Azeri buffaloes belong to 3B group. Both of Mazani and Azeri buffaloes are riverine. Based on the karyotype symmetry parameters, Mazani buffalo populations showed more symmetry that Azeri buffalo populations. The chromosomal evidences and other detailed karyotype analysis allows us to detect interrelationships of spices from a chromosomal point of view, to group the different populations and postulate relationships among them.