Molecular and Phylogenetic Analysis of Polymyxa betae Keskin, Isolated from Sugar Beet Fields in Razavi and Northern Khorasan Provinces by ITS Sequence Analysis

For study the genetic diversity and Phylogenetic analysis of Polymyxa betae Keskin، isolated from sugar beet fields in Razavi and Northern Khorasan provinces، soil of 49 fields were sampled and sugar beet seeds (variety IC) were planted that. After DNA extraction، a genomic region comprises of 463 bp of P. betae isolation including 18s r RNA، ITS1، 5. 8srRNA، ITS2 and 28s rRNA was amplified using Pxwd1 /ITS4 primers. The 22 isolates were selected and sequenced. According to our results، the fungal isolates grouped in two distinct clades. Isolates belonging to Razavi and Northern Khorasan provinces were clustered in one group with high similarity، separated from European group. Consequently our data indicated that the ITS2 region is more proper to analyse genetic variation among the P. betae isolates than ITS1region.