Isolation and Identification of Phosphorus Solubilizing Fungi in Forest Soils around Koh-Sepid Lar Using ITS-PCR Method

The present study was conducted to isolate and identify phosphorus solubilizing fungi and to evaluate their ability through a qualitative and quantitative experiment. An experiment was carried out with 5 soil samples of Koh- Sepid Lar, Kohgyloyeh and Boyer Ahmad province. The ability of isolates was studied in solid and liquid cultures. Quantitative experiment consisted of blank, four fungi isolates and Aspergilus niger, with 3 replications. Two fungi that showed the highest potential in P solubilization were used for identification by ITS- PCR methods. Four of the fungi produced very clear zone on the Pikovskaya culture. The order of soluble P content in the liquid culture was: blank< fungi 4< fungi 3< fungi 2< Aspergilus niger< fungi 1. Two high potential isolates, 1 and 2, were Cladosporium cladosporioides and Eupenicillium rubidurum, respectively. This is the first report about the potential of these fungi to solubilize P.