Genetic and phylogenetic analysis of D-loop region in camelus dromedaries and camelus bactrianus of Iran

Message:
Abstract:
Iranian native species are considered as part of the national asset therefore their preservation is important. Due to severe decrease in their population size in some parts of Iran, more attention to these species from conservation genetics perspective is required. The goal of this study was to analyse the DNA sequence of the D-loop region of mitochondria in Iranian camels and draw the phylogenetic tree. For this study 10 blood samples from Dromedary and 15 blood samples from Bacterian camels were collected. After DNA extraction, D-loop region was amplified with specific primers using PCR, and then the fragments were sequenced. In studied populations,3 and 6 haplotypes were observed in single-humped and double-humped Iranian camels, respectively. The Neighbor-Joining phylogenetic test results showed that Iranian camels have the lowest genetic distance with Arabian camels, it can be concluded that Iranian camels has genetic similarities with Arabian camels.
Language:
Persian
Published:
Journal of Ruminant Research, Volume:3 Issue: 2, 2015
Page:
93
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