Recombinant β-NGF production in E.coli using date syrup

Production of recombinant proteins such as β-NGF using prokaryotic hosts is the topic of many recent researches. However, bacterial cell culture media are cheaper than eukaryotic cell culture media, but in industrial production scale they are not cost effective at all for biotech companies. Therefore, survey to find inexpensive cell culture medium that bacterial cells not only can grow in it but also produce recombinant proteins is very important. In this study, for the first time date syrup and yeast extract mixture was used as an inexpensive medium. In RSM (response surface methodology) studies different concentrations of date syrup and yeast extract were used as carbon and nitrogen sources respectively. The results indicate that the 20 g/lit of carbon and 5 g/lit of nitrogen are optimum for bacterial growth. Also the data show that recombinant bacteria not only can grow but also can produce recombinant proteins such as β-NGF using this synthetic medium.