Assessment of Genetic Diversity Among and Within Different Types of Tobacco (Nicotianatabacum L.) Using IRAP and REMAPMarkers

In present study IRAP and REMAP markers were used to assess genetic diversity levels in 45 genotypes of different types of tobacco germplasm, including burley, flue-cured and oriental tobacco that out of 20 composition primers, 5 IRAP primersand 9 REMAP primers produced scorable and polymorphic banding patterns. Totally from 188 amplified loci, 153 loci were polymorph. RTR-10 and RTR-1 with 22 and 16 bands andUBC817+RTR-1 with 4 bands had the maximum and minimum polymorphic bands, respectively. In overall studied genotypes, PIC value varied between 0.16 to 0.33 and MI between 0.34 to 6.25. Cluster analysis using UPGMA method, 45 genotypes were placed in five cluster groups. GroupsI, II and III to V including burley type, flue-cured tobacco and oriental tobacco genotypes, respectively.Canonical Discriminate Function via Fisher's linear method was ableto confirm 73.3 percent of the validity of clustering analysis result.