Study and Detection of Ultra-Weak Photon Emission from HT-29 Cells under the Effect of Trypsin

In addition to chemical and electrical mechanisms, cell organisms communicate each other through electromagnetic waves, known as biophotons. Detection of these waves shows that when cell organisms are being dividing, injured, or dying, they emit more photons than when they are healthy. The purpose of this study was to detect the biophoton waves emitted from cancer cells (HT-29) affected by trypsin. Trypsin is a factor contributes to gradual death of cells. This study was carried out on HT-29 cells in three groups. In the first group, the cells were affected by trypsin. In the second group, the cells were not influenced by trypsin; and in the third group, an empty plate was used to measure the background radiation. For this purpose, the amount of the radiation emitted from all three groups was measured using photomultiplier tubes (PMT) apparatus and compared. Computation of the means and standard deviation of the whole surface of emitted biophotons demonstrated that the group containing trypsin significantly emitted more amounts of biophotons than the group without trypsin and the group considered for the measurement of the background radiation (P < 0.05). In addition, over the time, the amount of emitted biophotons increased. The results showed that when the cells were under the influence of trypsin, the amount of biophoton emissions increased. With the elapsed time, more cells were faced with the death threshold, therefore, the amount of emitted waves from the cells affected by trypsin increased significantly rather than the cells were not influenced by trypsin.