The effects of using rice bran, enzyme and probiotic on performance, egg quality traits and blood metabolites in laying hens

This experiment was conducted to evaluate the effects of using rice bran, combo multi-enzyme and probiotic (protexin) in diet on performance, egg traits, biochemical parameters and blood cells of commercial laying hens. This experiment carried out using 384 Hi-line (W36) laying hens in a completely randomized design as factorial (2×2×2) arrangement with two levels of rice bran (0 and 10%), two levels of combo multi-enzyme (0 and 0.05%) and two levels of protexin (0 and 0.005%) in 8 treatments, 4 replicates and 12 birds per replicate for 12 weeks (65-76 weeks). Using 10% rice bran in diets improved the egg weight, egg production percentage and egg mass, feed conversion ratio, egg yolk color index and blood HDL of laying hens (P<0.05), whereas did not have any significant effects on the amounts of feed intake and immune cells (P>0.05). Combo multi-enzyme in diets did not have any significant effects on performance, egg quality traits and blood parameters of laying hens (P>0.05). Using protexin in laying hens diets without having any significant effects on egg quality traits and blood parameters (P>0.05), improved the feed conversion ratio (P<0.05). In using rice bran with combo multi-enzyme and protexin, performance, egg traits and blood HDL improved (P<0.05), whereas did have any significant effects on the amounts of feed intake and immune cells (P>0.05). The overall results indicated that in laying using 10% rice bran with 0.05% of combo multi-enzyme and 0.005% of probiotic can improve the performance, egg traits and blood HDL.