Cytotoxic Activity of Natural Copmponents Soluble in Methanol and Diethyl ether of Dysidea pallescens from Hengam Island, Persian Gulf

Sponges are the most primitive of the multicellular, These organisms don’t have any mechanical defense system, so their early appearance in evolution has given them a lot of time for the development of advanced secondary metabolites as chemical defense system. Sponges have the potential to provide drugs from chemical components against diseases. In this investigation the sponge samples, which it is Dysidea pallescens, were collected at depth of 15- 20 meter, from locations on the coastline of Island Hengam in Persian Gulf of Iran. For identifying natural components, methanol and diethyletter were used as extraction solvents, after removal of the solvents, the in vitro cytotoxic activity was identified. In vitro cytotoxity screening, by XTT assay, against KB/ C152 and HUT-78/ C185 cell line, was conducted in this study in 1 - 500 μg/ml . IC50 for diethyletter and methanol extract was 200 μg/ml in HUT-78 , IC50 for diethyletter extract was 325μg/ml and methanol extract 325μg/ml in KB.