Isolation of Lipase producing bacteria from olive and the various parameter effect on enzyme production in solid state fermentation

An aerobic, meophilic and lipolitic Bacillus.sp bacterium (lipase activity 40 U/ml) was isolated from the olive extract. This isolate was identified based on morphological and biochemical characterization and 16S rRNA gene sequence. Effect of the solid substrate (pomegranate seed ground, grape seed ground and coriander seed ground as a solid substrate), the substrate particle size, moisture content and rotation speed as factors affecting the solid state fermentation was studied.The optimum lipase activity (120 U/ml) was achieved at optimum levels of factors of coriander seed extract (3.0w/w), olive oil concentration (25 g/L), yeast extract (10 g/l), moisture content (70.0%), and agitation rate (170.0rpm) that lipase activity increased to 3.07-fold.