Isolation and molecular identification of Mycoplasma pulmonis from the respiratory tract of Rattus rattus

Mycoplasma is the smallest self-replicating bacteria polymorphic are having a diameter of about 200-500 nm, and lees cell wall. Because of their small size and flexibility to easily pass through the filter membrane and contaminate represented. These organisms are unique in nature, the wide range of diseases among animals, especially respiratory & genitalia diseases were among the rodents.The aim this study was to isolate and identify the molecule Mycoplasma of Rats.
With aim to isolation of mycoplasmas pulmonis, the tracheal samples were collected and cultured on the medium 243 and Microaerophilic was placed. Genomic DNA was isolated from bacteria grown. 16srRNA gene amplified with PCR. The PCR products, finally to identify the bacteria was purified, sequenced were a BLAST.
Macroscopic studies on the isolated colonies showed fried egg colonies were established. Based on the 16SrRNA sequencing studies, this bacterium belonging Mycoplasma genus & had 99% similarity to Mycoplasma pulmonis.
Due to the lack of similarity of 100%, a new strain of bacterium, Mycoplasma pulmonis strain IRMT179 name were called. Finally, the gene bank was recorded with acceptation number KP836312. This study is the first time in Iran; Mycoplasma pulmonis was isolated from the respiratory tract an urban rat.