Detection production process of defense enzymes in two tomato cultivars against root-knot nematode Meloidogyne incognita race 2 in greenhouse conditions
To investigate the effect of root-knot nematode M. incognita race2 on tomato defense enzyme production process, an experiment was conducted under greenhouse conditions. The experiment was carried out in a completely randomized split split plot design with two susceptible (Falat Y) and tolerant (Gina VF) tomatoe varieties with four nematode population levels of 0 (control), 500, 1000 and 2000, the number of larvae of second stage with four different sampling times (0 (control), 3, 5 and 7 days after inoculation) and with four replications. In this experiment, after sampling, purification, identification and propagation of nematode species and race of nematodes were determined. With nematode purified amplification on the Rutgers cultivar tomato inoculum was obtained. The four-leaf stages were inoculated population in levels and the time mentioned of the enzyme was measured. The results showed that increasing levels of nematode population affected plant biochemical activities and the total protein content decreased. The activity of catalase, peroxidase, polyphenol oxidase and phenylalanine ammonia lyase involved in the defense reaction of host plant increased. The highest rates were observed in the catalase enzyme and in the tolerant variety. In the presence of nematode stress, plant activated its defense mechanism that has enabled it to increase its enzymatic activity which was at its maximum value 5 days after inoculation.