Effect of Temperature Ppretreatment on Callogenesis and Gametic Embryogenesis in Anther Culture of Cucumber
Author(s):
Abstract:
In this research, the effect of temperature pretreatment on callogenesis and gametic emberyogenesis resulting from anther culture of cucumber (Cucumis sativus L.) using a local variety of cucumber (Esfahani) and an Italian cultivar (Beta Alpha) was investigated. In the first experiment, cold pretreatment (control, 4 and 10°C for two and four days) was considered as main plot and the two cultivars as sub-plot. In the second experiment, temperature (control, 30 and 35°C for two and four day) was considered as main plot and two cultivars as sub-plot. Results showed significant differences among the various types of temperature pretreatments for callogenesis and gametic embryogenesis resulting from anther cultures of two cucumber cultivars. In the first experiment, using 4°C cold pretreatment for 4 days showed the highest callogenesis and mean number of embryos per anther, in anther culture of Esfahani and Beta Alpha cucumbers, respectively. In the second experiment, application of heat pretreatments of 30°C for 4 days, and 30°C for 2 days resulted in the highest callogenesis and mean number of embryos per anther in anther culture of Esfahani cucumber. Furthermore, heat pretreatment of 30°C for 2 days in Esfehani cucumber showed the highest direct embryogenesis.
Keywords:
Language:
Persian
Published:
Journal of Horticultural Science and Technology, Volume:16 Issue: 1, 2015
Pages:
149 to 160
magiran.com/p1532290
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!