Control of stress responses by Lactobacillus rhamnosus LX-108 in male wistar rats under restraint stress condition

Stress and its role in amplifying symptoms of disease, is one of the most important problem of mankind in developed counties. By identification of the roles of microflora in brains function, it seems that probiotics could be used for control and ameliorating of brain responses such as stress. So in this study, the effect of Lactobacillus rhamnosus LX-108, in ACTH secretion level was investigated in male wistar rats under chronic restraint stress (CRS). In this research 24 male wistar rats weighing 180- 200 gr randomly divided in to 3 groups: Negative control, Positive control (received CRS), and trail group (orally gavaged with 1 ml of probiotic suspension and received CRS). Rat’s weight was measured every week and animals were anesthetized with ether after three weeks, and blood samples were obtained directly from their hearts. ACTH concentrations of sera were measured by ELISA method with special kit. Indeed microflora profile was identified in fecal samples. Our results showed that administration of LX-108 can inhibit the induction of ACTH under CRS condition. Besides this probiotic resulted in increasing of intestinal Lactic acid bacteria and decreasing the Enterobacteriaceae level which induced in CRS condition. Fortunately administering of this probiotic inhibit weight loss induced in CRS condition. After clinical trials, Lactobacillus rhamnosus LX-108 and its probiotic products can be used for controlling of stress and its distracutive effects.