Protective effect of melatonin in lamotrigine-treatment on newborn mice

The purpose of this study was to investigate the Protective effect of melatonin in lamotriginetreatment on newborn mice cerebellum. The adult female mice were divided randomly into 5 groups, including one control and four experimental groups, after formation vaginal plagues. Group I received normal saline, group II received low-dose the lamotrigine, group III received low-dose lamotrigine melatonin, Group IV received high dose lamotrigine, and group V received high doses of lamotrigine melatonin on days 9, 10 and 11 of gestation. They received the drugs within gavages. Babies born after morphometric procedures were placed within the 10% fixative solution for hystothecnic procedures. The Samples were stained with H&E general staining and special staining of Tunnel. The data were analyzed statistically using ANOVA followed by the Tokey’s multiple comparison tests was performed. The results of microscopic studies demonstrated tissue changes including incomplete villus in the cerebellum, lack of completion of cerebellar cortex’s 3 layers, the presence of apoptosis or necrosis and congestion in the Lamotrigine intervention groups. Mean while, the combined effect of melatonin lamotrigine was significant compared with only lamotrigine group. Based on the obtained results it was observed in the fetus growth parameters morphometry that the effect of melatonin is a protective effect as an antioxidant against free radicals created from administrating of Lamotrigine. Also, it was observed that melatonin had no protective effect on decreasing of gray layer thickness undertreated with Lamotrigine. The density of Purkinje cells was decreased because of reduced mitotic activity in the intervention groups; so, the incidence of apoptosis was observed. Whereas the combined effect of melatonin in the intervention groups had no protective effect on the incidence of apoptosis. Prescription Lamotrigine reduced fetal growth parameters and Parameters will be slightly reduced cerebellar tissue. Melatonin administration significantly from the beginning of gestation, fetal growth parameters has an effect, but a significant protective role in tissue pathologic changes in the cerebellum and cerebellar tissue has no character.