The evaluation of antibacterial activity of TiO2 nanoparticles, EDTA and garlic on standard strain of Pseudomonas aeroginusa by microdilution method

Importance of microbial contamination in food industrial is always important health problem especially in high risk population such as children and old individual. TiO2 nanoparticles were obtained through the hydrolysis of TiCl4 (Titanium tetrachloride).It was slowly added into distilled water under constant rotation for 5 hours. This Solution was dried at the 80-100 0C and calcinated at the 5500C. Size and type of these Nanoparticles were characterized by scanning electron microscope (SEM) and X-Ray-: Diffraction (XRD) pseudomonas aeroginusa strain (ATCC 27853) were cultured on nutrient agar medium (NA) for 24h at 370C. Suspension by concentration 1×106 cells/ml was prepared. Minimum Inhibitory Concentration (MIC) test for TiO2 and EDTA and garlic were evaluated. Evaluation of Morphology and diameter of the TiO2 nanoparticles with SEM showed that nanoparticles were spherical with diameter between 40-65nm. MIC of TiO2 and EDTA and garlic on pseudomonas aeroginusa ATCC27853 were 2/2, 24/92 and 40 μg/ml respectively. In this study we synthesized the TiO2 nanoparticles with chemical method and showed that it has antimicrobial activity compared with garlic, EDTA and positive control, so it could eliminate Pseudomonas aeroginusa in field of food industrial specially in packaging.