STADY OF HYPERRICUM PERFORATUM L. HYDROETHANOLIC LEAF S EXTRACT ON some BLOOD PARAMETERS IN MALE RATS TREATED BY CYCLOPHOSPHAMIDE

Cyclophosphamide is an anticancer drug which causes alkylation on DNA in cells. The side effects of cyclophosphamide are bone marrow damages and anemia. Hypericum perforatum is a medicinal plant which widely used in traditional medicine. In this study the hemotopoetic effect of Hypericum perforatum leaf extract (HPE) on bone marrow function and blood parameters in male rat were treated with cyclophosphamide was investigated.
Method & Materials: The 42 male Wistar rats with 220±25 gr body weight were divided randomly in 6 groups (n=7): control (taking normal saline,0.5ml/day, i,p ),witness group taking cyclophosphamide (15mg/kg/day, i.p.),positive control group taking HPE (200mg/kg, i.p/day), treated groups 1,2 and 3: (taking cyclophosphamide ,15mg/kg/day, i.p. 100mg/Kg, 200mg/Kg and 400mg/kg HPE /day for 10 days, i,p). After the examination the blood samples were collected from heart directly and RBC, WBC, Hct, Hb and PL were analyzed. All data were expressed as mean±SEM and statistical significance differences was accepted at P Our results showed that the cyclophosphamide has injury effect on bone marrow tissue. The blood cells count decreased in groups treated by cyclophosphamide significantly. The groups treated with HPE were significantly increased in blood parameters compared with group taking cyclophosphamide alone (P The Hypericum perforatum hydoethanolic extract has antioxidant and flavonoids compounds which can protect the bone marrow tissues against cyclophosphamide