Evaluation of Different Combination of Trichoderma Species for Controlling Fusarium Rot of Chickpea

The Fusarium disease, caused by Fusarium oxysporum and F. solani has been observed in different areas of Iran in recent years. Current biocontrol studies have confirmed the effectiveness of the Trichoderma species against many fungal phytopathogens. In this study, biocontrol effects of Trichoderma isolates, both separately and their combined effects were evaluated against Fusarium oxysporum, F.solani pathogen. In this investigation the ability of Trichoderma harzianum and T.asperellum isolates, isolated from soil and chickpea root, were compared with combination of three fungal lentil Fusarium rot. Three isolates (Trichoderma) of isolates T1, and T2 around Fusarium oxysporum and F.solani hyphae. Volatile metabolites of all isolates reduced the mycelial growth of fusarium diseas pathogen. T1 and T2 isolates and their combination were more effective than other treatments to control the disease, where it reduced 75 to 78% disease. All of treatments showed significant differences with control (Trichoderma harzianum)T1, (Trichoderma asperellum)T2, were selected before test (shown good antagonist effect) after screening tests for antifungal combination effects against Fusarium disease pathogen in greenhouse. In dual culture tests, three (T1, T2) isolates covered and colonized the colony of the pathogen. In this research three (T1, T2) isolates covered and colonized the colony of the other Trichoderma isolates. Microscopic studies revealed hyphal coiling (hyperparasitismplants).